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Fig. 2 | BMC Cancer

Fig. 2

From: Evaluation of KIF23 variant 1 expression and relevance as a novel prognostic factor in patients with hepatocellular carcinoma

Fig. 2

Characterization of anti-KIF23 V1 antibody. a HEK293T cells were transiently transfected with pRK-FLAG-KIF23 V1, pRK-FLAG-KIF23 V2, or pRK-FLAG plasmids and the protein expression was detected by Western blotting employing anit-KIF23 V1 antibody. b Western blotting analysis of the same samples as in (a) was performed with commercial anti-KIF23 antibody. c HLE cells were transfected with either control or KIF23 V1 siRNAs and whole cell extracts were processed for immunoblotting with anti-KIF23 V1 antibody. d Western blotting analysis of the same samples as in (c) was performed with commercial anti-KIF23 antibody. All the membranes were reblotted for the expression of tubulin

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