Fig. 4

Histone deacetylase inhibitors caussed apoptotic cell death in ATC and PTC cells. Immunoblot analyses suggested that the indicated inhibitors increased the levels of apoptotic proteins and reduced those of anti-apoptotic proteins in ATC and PTC cells (a). An electrophoretic mobility shift assay was carried out using a ³²P-labeled oligonucleotide probe for the NF-κB binding sites on the Bcl-2 promoter (b). TUNEL assay of ATC and PTC cells; TUNEL-positive (apoptotic) cells are indicated (× 400) (c, d)