Fig. 3

Histone deacetylase inhibitors induced cell cycle arrest and endoplasmic reticulum stress in ATC and PTC cells. Immunoblot analysis of the indicated cell lines following exposure to SAHA, TSA or HNHA showed that HNHA potently induced the expression of cell cycle arrest proteins and reduced expression of positive regulators of the cell cycle (a). SNU-80 and SNU-790 were exposed to the indicated inhibitors for 24 h prior to analyzing the expression of GRP 78, ATF 4, CHOP, PERK, p-PERK, eIF2α and p-eIF2α (markers of endoplasmic reticulum stress) by immunoblotting (b). Cells were exposed to the indicated inhibitors, harvested and stained with propidium iodide prior to analysis by flow cytometry and FlowJo v8 software (c and d)