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Fig. 1 | BMC Cancer

Fig. 1

From: Expression of mouse CD47 on human cancer cells profoundly increases tumor metastasis in murine models

Fig. 1

Construction and expression of mCD47. a Schematic illustration of mCD47-GFP-Luc and GFP-Luc in a transposon vector construct. The coding sequences for murine CD47 (mCD47) or GFP-luciferase fusion gene (GFP-Luc) are shown. The locations of the promoter, the polyA signal, and the internal ribosome entry site (IRES) are indicated. b Transfection efficiency of mCD47. PC-3 cells were transfected with a Piggyback transposon with mCD47- GFP-Luc or GFP-Luc vectors, or mock transfected. Following puromycin selection, cells were stained with an isotype or a PE-conjugated anti-mCD47 antibody, and then analyzed by flow cytometry for detection of PE and GFP

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