Fig. 4
Loss of MMP and caspase activation by BPIQ. a H1299 cells were exposed to media containing the indicated concentrations of BPIQ or vehicle control for 24 h, stained with DiOC2(3), then analyzed for changes in their fluorescent profile by flow cytometry. b Quantitative analysis. Data are presented as means ± S.D. Histograms represent one of three independent experiments. *p < 0.05 and **p < 0.001 against vehicle control, respectively. c Western blot analysis demonstrating BPIQ-induced cytochrome c release and cleavage of caspase-9 and −3, as well as PARP. β-actin was measured as an internal control. d The distribution of cytochrome c in the cytosol of two NSCLC cell lines A549 and H1299 following 2 μM BPIQ treatment. ▪ mitochondria; ▪ cytochrome c; ▪ DAPI; ▪ co-localization of mitochondria and cytochrome c. Magnification 200 x