Fig. 5

Flavone, apigenin and luteolin regulate the expression of the cyclin-dependent kinase inhibitors p21 and p27 through the Akt-FOXO3a signaling axis. a Real-time PCR for p21 and p27 expression in untreated MCF-7 cells (control) and cell treated with the IC₅₀ concentrations (Table 1) of flavone, apigenin, and luteolin for 0 and 24 h. b Western blot analyses of total p21 in cytoplasmic and nuclear extracts isolated from MCF-7 cells treated with the IC₅₀ concentrations (Table 1) of flavone, apigenin, and luteolin from 0 to 48 h. c Densitometric quantification of the p21 expression from the western blot analyses. d Western blot analyses of total p27 in cytoplasmic and nuclear extracts isolated from MCF-7 cells treated with the IC₅₀ concentrations (Table 1) of flavone, apigenin and luteolin for 0–48 h. e Densitometric quantification of p27 expression from western blot analyses. Loading control (B and C): cytoplasmic, β-actin: nuclear proliferating cell nuclear antigen (PCNA). Results are the mean ± standard deviation of three independent experiments. P < 0.05 is considered as statistically significant. Symbols: *: P < 0.05; #: P < 0.01; ★: P < 0.001