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Fig. 4 | BMC Cancer

Fig. 4

From: Flavones inhibit breast cancer proliferation through the Akt/FOXO3a signaling pathway

Fig. 4

Flavone, apigenin, and luteolin activate FOXO3a, which is associated with a change in the signal transduction pathway. a Real-time PCR for FOXO3a. HS578T, MDA-MB-231 and MCF-7 cells were treated with the IC50 concentrations (Table 1) of flavone, apigenin, and luteolin for 0 h (control) and 24 h. b Western blot analyses of total FOXO3a in cytoplasmic and nuclear extracts isolated from MCF-7 cells treated with the IC50 concentrations (Table 1) of flavone, apigenin, and luteolin for various time from 0 to 48 h. Loading controls: cytoplasmic, β-actin; nuclear, proliferating cell nuclear antigen (PCNA). c Densitometric quantification of the FOXO3a expression from the western blot analyses. d MCF-7 cells were treated with IC50 concentrations (Table 1) of flavone, apigenin, and luteolin for 0–48 h. Western blot analyses of the total protein kinase B (Akt) and Ser-473-phosphorylated Akt (p-Akt) in cytoplasmic and nuclear extracts isolated from MCF-7 cells treated with the IC50 concentrations (Table 1) of flavone, apigenin, and luteolin for 0–48 h. Loading control: β-actin. e Densitometric quantification of the Akt and Ser-473-phosphorylated Akt expression from the western blot analyses. Results are the mean ± standard deviation of three independent experiments. P < 0.05 is considered as statistically significant. Symbols: *: P < 0.05; #: P < 0.01; : P < 0.001

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