Table 1 RT-PCR Primers
From: RUNX1-Evi-1 fusion gene inhibited differentiation and apoptosis in myelopoiesis: an in vivo study
Name | Forward primer | Reverse primer | Product (bp) |
|---|---|---|---|
RUNX1-Evi-1 | ATATCGCTGCGAAGACTGTGACCA | TGAAGGTTGCTAGGGTCCGTGAAA | 381 |
scl | GAACAGTATGGGATGTATCCTAGC | CGTTGAGGAGCTTAGCCAGA | 265 |
lmo2 | ACACTGGAGGCAAATGAGGAG | AGTAAAGCCTGCGTCCCACC | 193 |
gata2 | CTGCCAGACAACCACGACC | CCAGATCGTTTACTCCTCTTGG | 180 |
gata1 | CCATCGTATTATTCCACCAGC | GGATGTGGGGTTGTAGGGAG | 159 |
pu.1 | TCCCAGCAGTCGTAGTCCTC | CCATTTCGCAGAAGGTCAA | 141 |
mpo | GGGTTGACCACGATCTGACT | CAGGGAGACAGGTGTTAGGG | 159 |
c-myb | GGCAGAAAGTCCTCAACCC | ATCGGTTTCCAAGTTTCTCG | 252 |
runx1 | TGGGACGCCAAATACGAAC | AGGACGGAGCAGAGGAAGTT | 227 |
P21 | GAACGATGTGCTGCACTCCC | TGTCAATAACGCTGCTACGAGAC | 236 |
P27 | CGACTGTAGGGTAACGGAGCA | GGGTGTCGGACTCAATGGTT | 178 |
P53 | CGGCGATCATGGATTTAGGC | TTCAGCCACATGCTCGGACT | 202 |
ras | GTCCACGATGAATCCCGAATA | TCTCCTGCCCTGCTGTATCC | 212 |
Bax | ACAGGGATGCTGAAGTGACC | GAAAAGCGCCACAACTCTTC | 236 |
Bcl2 | AACGCGAATTTGAGGAAATG | TATCTACCTGGGACGCCATC | 189 |
JNK | AACAGGAATAAGCGCGAGAA | TTGTGGTTGACGCATTTCAT | 245 |
JUN | AAACAGCGCTTTCTCTCAGC | TGATCATGCCGTTGCTAGAC | 221 |
skp2 | ATCTGGGACTGAGCCGTTGT | GAGAACGGCTGCGTGTTGAT | 166 |
Caspas3 | AGGCTTGTCGAGGAACAGAA | CATGATCTGCAAGAGCTCCA | 233 |
smad7 | GGTTCTGTGCCTGCTTCCA | TGCCCTGAGGTAGGTCGTAGA | 202 |
smad4 | AGACCTCCACATACCACCACA | GTCCATCTCGAAGTAGGCAAT | 221 |
β-actin | CCTGACCGAGAGAGGCTACA | CGCAAGATTCCATACCCAAG | 242 |