Fig. 3
From: RUNX1-Evi-1 fusion gene inhibited differentiation and apoptosis in myelopoiesis: an in vivo study
Cytological analysis of Tg(RE:HSE:EGFP) zebrafish. Cytology of hematopoietic cells from WT (a) and Tg(RE:HSE:EGFP) F2 generation (b) zebrafish at 60 dpf. The blood cells from WT fish were predominantly erythrocytes, and by contrast, erythrocytes were significantly inhibited in Tg(RE:HSE:EGFP) fish, enriched for abundant blast-like cells, which are larger than the erythrocytes and have high nuclear to cytoplasmic ratios, containing multiple large nucleoli (black arrow). These blasts were similar to that of human AML peripheral blood. The similar feature presented in single cell suspensions of kidneys from WT (c) and Tg F2 generation (d)