Fig. 4

Sulindac sulfide decreases promoter gene activity of Sp1, Sp3, survivin and VEGF in colon cancer cells. a Cells were transfected with promoter-luciferase reporter constructs containing −751 bp promoter sequence of SP1 gene (pSp1-Luc) or −417 bp of SP3 gene (pSp3-Luc). b Cells were transfected with constructs containing −269 bp of BIRC5 gene (pSurvivin-Luc) or −2018 bp of VEGFA gene (pVEGF-Luc). After 6 h of transfection, cells were treated with DMSO, 25, 50 and 100 μM sulindac sulfide for 13 h. Luciferase activity was determined as described under Materials and Methods. Experiments were done in triplicate and results are expressed as fold of control (mean ± S.D.). Asterisk (*) indicates statistical difference between control (DMSO) and treatment (P < 0.05)