Fig. 3

Sulindac sulfide decreases expression of EGFR, p65, VEGF and survivin and induces PARP cleavage in colon cancer cells. a, b Sulindac sulfide decreases expression of EGFR and the p65 subunit of NFκB in SW480 and RKO cells. Cells were treated with DMSO, 25, 50, 75 and 100 μM sulindac sulfide for 24 and 48 h. EGFR and p65 cellular levels were determined by Western blot analysis as described under Materials and Methods and β-actin was used as loading control. c, d Sulindac sulfide decreases expression of VEGF and survivin and induces PARP cleavage in SW480 and RKO cells. Cells were treated with DMSO, 25, 50, 75 and 100 μM sulindac sulfide for 24 and 48 h. VEGF, survivin and cleaved PARP cellular levels were determined by Western blot analysis as described under Materials and Methods and β-actin was used as loading control