Fig. 5

Inhibition of STAT3 phosphorylation prevents the Hyper-IL-6-mediated increase of CEACAM expression. a, HT29p cells were seeded in 6-well plates, serum-starved overnight and pre-treated with FLLL31 (5 μM) for 2 h. Subsequently, cells were treated with serum-free medium with or without Hyper-IL-6 (15 ng/ml). After 15 min, cells were lysed and the lysates analyzed for the phosphorylation of STAT3 in Western blots. b, After 24 h, RNA was isolated and analyzed by qPCR for the expression of CEACAM5 and CEACAM6. PPIC and RPL22 were used as reference genes