Fig. 3
From: Immunohistochemical evaluation of ROCK activation in invasive breast cancer
Validation of anti-pS1366 ROCKII antibody specificity in ROCKII depleted cell block. HEK293T cells were transiently transfected with or without siRNA targeting human ROCKII for 2 days and then transfected with or without pEGFP-RhoAV14 for 16 h. (a) Cells were pelleted and fixed. The samples from these cell blocks were stained with anti-pS1366 ROCKII antibody. Scale bar, 20 μm. (b) Cells were harvested for Western blotting with antibodies as indicated