Fig. 2
From: A novel human leiomyoma tissue derived matrix for cell culture studies
Colony formation and hanging drop spheroid culture of HSC-3 cells within Myogel combined with LMA and plain LMA. Colonies were photographed with 10x and 40x objectives (a). Cell numbers per colony were counted from the photographs (b). Spheroids were photographed with 4x objective (c). Spheroid area in ratio to 0 h spheroid area was calculated after 24 h, 48 h and 72 h incubations (d). Altogether 47 colonies were calculated from four Myogel-LMA and 50 colonies from four LMA wells in two independent experiments. Enlargement of altogether five spheroids in both Myogel-LMA and LMA was followed in two independent experiments