Fig. 3

TSG101 depletion enhances MMP-9 mRNA expression in HT1080 cells. a. MMP-related gene mRNA expression. Subconfluent cells were serum starved for 16 h and subsequently incubated in fresh serum-free medium containing or not 200 nM PMA for 7 h. RT-PCR was performed to examine levels of the indicated mRNAs. The ratio of each MMP-related gene mRNA level relative to the GAPDH mRNA level is expressed as arbitraryunits. Each MMP-related gene level in non-treated cells is set to 1.0. b. MMP-related gene expression in TSG101-depleted cells. Cells transfected with control (con) or TSG101 (TSG#1 or #2) siRNA were incubated in fresh serum-free medium containing or not 200 nM PMA for 7 h. RT-PCR was performed to examine levels of the indicated mRNAs. The ratio of each MMP-related gene mRNA level relative to the GAPDH mRNA level is expressed as arbitrary units. Each MMP-related gene mRNA level in the cells transfected with control (con) siRNA is set to 1.0 individually in non- and PMA-treated cells. The results shown are the means ± S.D. of three independent experiments. * p < 0.05; **, p < 0.005; ns, not significant, by a Student’s t-test