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Fig. 2 | BMC Cancer

Fig. 2

From: TSG101, a tumor susceptibility gene, bidirectionally modulates cell invasion through regulating MMP-9 mRNA expression

Fig. 2

TSG101 depletion leads to increased secretion and expression of MMP-9 in HT1080 cells. a. MMP-9 secretion in TSG101-depleted cells. bc. MMP-9 expression in TSG101-depleted cells. Cells transfected with control (con) or TSG101 (TSG#1 or #2) siRNA were incubated in fresh serum-free medium containing or not 200 nM PMA for 7 h. MMPs in conditioned media (a, top) and cell lysates (b, top) were measured using gelatin zymography. The amounts of total MMP-2 (MMP-2 + active-MMP-2) and MMP-9 in the conditioned media (a, bottom) and cell lysates (b, bottom) of PMA-treated cells were quantified and are expressed as arbitrary units. The amount of each MMP in the conditioned media and cell lysates of the cells transfected with control (con) siRNA is individually set to 1.0. Cell lysates were analyzed by western blot using the indicated antibodies (c, left). The amount of MMP-9 in PMA-treated cells was quantified and is expressed as arbitrary units (c, right). The amount of MMP-9 in the cells transfected with control (con) siRNA is set to 1.0. The blots and gels shown are representative of three independent experiments. The results shown are the means ± S.D. of three independent experiments. **, p < 0.005; ns, not significant, by a Student’s t-test

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