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Fig. 1 | BMC Cancer

Fig. 1

From: TSG101, a tumor susceptibility gene, bidirectionally modulates cell invasion through regulating MMP-9 mRNA expression

Fig. 1

TSG101 depletion promotes cell invasion of HT1080 cells. a. Depletion of TSG101 by siRNA. Total cell lysates of cells transfected with control (con) or TSG101 (TSG#1 or #2) siRNA were analyzed by western blot using the indicated antibodies. bc. Cell migration of TSG101-depleted cells. Confluent cells transfected with control (con) or TSG101 (TSG#1 or #2) siRNA were scratched and incubated in fresh serum-free medium for 3 h (b). Scale bars, 200 μm. Cell migration into the wound area in (b) was quantified (c). Relative migration activities of the cells transfected with TSG101 (TSG#1 or #2) siRNA are expressed as the proportion of migration of the cells transfected with control (con) siRNA. de. Cell invasion of TSG101-depleted cells. Cells transfected with control (con) or TSG101 (TSG#1 or #2) siRNA were allowed to invade for 18 h (d). Scale bars, 200 μm. Cell invasion through the filter in (d) was quantified (e). Relative invasion activities of the cells transfected with TSG101 (TSG#1 or #2) siRNA are expressed as the proportion of infiltration of the cells transfected with control (con) siRNA. The blots and images shown are representative of three independent experiments. The results shown are the means ± S.D. of three independent experiments. * p < 0.05; **, p < 0.005; ns, not significant, by a Student’s t-test

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