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Fig. 3 | BMC Cancer

Fig. 3

From: Gonadotropin-mediated chemoresistance: Delineation of molecular pathways and targets

Fig. 3

hCG increases mRNA and expression levels of molecules associated with chemo-resistance, apoptosis and cell stress in tumor cells. a Semi-quantitative RT-PCR of molecules associated with chemo-resistance upon incubation of ChaGo-K-1 cells with medium or medium supplemented with hCG. β-ACTIN was employed as loading control. b Western blot of molecules associated with chemo-resistance upon incubation of ChaGo-K-1 cells with medium or medium supplemented with hCG. β-ACTIN was employed as loading control. c Flow cytometric analysis of molecules associated with chemo-resistance upon incubation of ChaGo-K-1 cells with medium or medium supplemented with hCG. Cells were permeabilized and stained with the respective antibodies. Filled red profiles indicate secondary antibody controls, green profiles indicate control cells and blue profile cells incubated with hCG. Data in A-C representative of four independent experiments. d Array dot blot analysis on ChaGo-K-1 cells incubated with medium, hCG, curcumin, or with hCG + curcumin. Expression levels of proteins associated with inhibition of apoptosis, chemo-resistance and protection from cell stress are shown. Cytochrome c (CYT C) was employed as loading control

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