Fig. 3

Fluorescence microscopy. Macrophages, MCF-7/GFP cells and MCF-7/macrophage hybrids, created by spontaneous cell fusion between GFP-labeled MCF-7 breast cancer cells and M2 macrophages were stained with an α-CD163 antibody and DAPI, and analyzed by fluorescence microscopy. Cells stained with secondary antibody only were used as negative control for CD163. The hybrids show cytoplasmatic expression of macrophage-specific antigen CD163 (red) and GFP, which are inherited traits from both maternal cells. Bars = 20 μm