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Fig. 1 | BMC Cancer

Fig. 1

From: Pan-Bcl-2 inhibitor Obatoclax is a potent late stage autophagy inhibitor in colorectal cancer cells independent of canonical autophagy signaling

Fig. 1

Obatoclax blocks cell growth and induces apoptosis in starving CRC cells. a and b HT29 and SW480 cells were treated with obatoclax in full supplemented medium or starvation as indicated. a Cell growth was assessed by MTT-Assay after 48 h. Cell death was assessed by Flow Cytometry after 48 h. Assays were done in triplicates. Values are expressed as mean ± SD. b Representative Western blots for p62 and LC3 I/II in HT29 and SW480 treated with obatoclax or ABT-737 in full supplemented medium as indicated. c Rel. mRNA levels of LC3 in HT29 cells after 24 h treatment with obatoclax. mRNA levels were quantified by qRT-PCR and normalized to GAPDH as housekeeping gene. Assays are representative of at least three independent experiments . ** = p < 0.01; *** = p < 0.001

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BMC Cancer

ISSN: 1471-2407

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