Diacerein retards cell growth of chondrosarcoma cells at the G2/M cell cycle checkpoint via cyclin B1/CDK1 and CDK2 downregulation

Table 1 Cell cycle distribution of chondrosarcoma cell lines after 24 and 48 h exposure to different concentrations of diacerein^a

Cell line	Treatment	h	G0/G1 (%)	S (%)	G2/M (%)
Cal-78	control	24 h	57.48 ± 8.19	23.79 ± 5.84	18.73 ± 2.51
	diacerein 30 μM	24 h	57.57 ± 8.30	25.23 ± 4.69	17.22 ± 3.68
	diacerein 100 μM	24 h	62.04 ± 5.54	22.01 ± 2.64	15.94 ± 2.85
	diacerein 300 μM	24 h	54.15 ± 7.33	26.76 ± 8.21	19.08 ± 3.67
	control	48 h	61.08 ± 3.03	21.23 ± 2.31	17.68 ± 0.89
	diacerein 30 μM	48 h	61.31 ± 1.16	23.66 ± 2.55	15.03 ± 1.80
	diacerein 100 μM	48 h	69.42 ± 2.80*	17.79 ± 2.38	12.78 ± 3.92
	diacerein 300 μM	48 h	56.95 ± 12.37	24.51 ± 9.02	18.53 ± 4.09
SW-1353	control	24 h	51.14 ± 7.05	22.73 ± 6.01	26.12 ± 1.35
	diacerein 30 μM	24 h	49.05 ± 5.46	27.15 ± 6.08	23.79 ± 1.23
	diacerein 100 μM	24 h	55.95 ± 4.11	19.53 ± 6.81	24.52 ± 6.83
	diacerein 300 μM	24 h	45.32 ± 12.41	25.35 ± 8.41	29.33 ± 7.06
	control	48 h	64.77 ± 7.67	15.65 ± 6.65	19.56 ± 1.03
	diacerein 30 μM	48 h	65.54 ± 5.75	15.37 ± 5.80	19.08 ± 0.31
	diacerein 100 μM	48 h	54.36 ± 5.92	22.16 ± 10.54	23.47 ± 4.86
	diacerein 300 μM	48 h	44.38 ± 10.52*	25.73 ± 7.23	29.89 ± 5.33*

^aAsterisks represent significant differences between control and treated cells (p < 0.0.5; n = 3; mean ± S.D.)

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