Fig. 7

Differential expression of TFIIIB subunits in female and male mice fed high or low isoflavone diets. Five week C57CBL/6 J (a) female and (b) male mice had free access to isoflavone-free, casein-based diet 5 K96 or regular Lab Diet 5001, with high isoflavone content. Post-treatments, total RNA was isolated from blood collected by cardiac puncture and analyzed by qRT-PCR for expression of BRF2, BRF1, BDP1, and TBP. The ΔΔCt method with GAPDH expression levels was used as a reference for normalization. Data presented are average of six female and six male mice from respective groups. Statistical analysis was performed using one-way ANOVA with a Tukey’s post-test with a 95 % confidence interval (Graphpad Prism 3.03); * = p <0.05; ** = p < 0.01; *** = p < 0.001