Fig. 1
From: Induction of proto-oncogene BRF2 in breast cancer cells by the dietary soybean isoflavone daidzein
Daidzein induces BRF1 and BRF2 mRNA specifically in MCF-7 breast cancer cells. MCF-7 (a) and MDA-MB-231 (b) cells were treated with 0, 3 or 10 μM daidzein for 48 h. BRF2, BRF1, BDP1 and TBP mRNA expression was then analysed by qRT-PCRusing the ΔΔCt method with RPS13 expression levels as a reference for normalization. Meta-analysis of three independent experiments performed in triplicate was completed using one-way ANOVA with a Tukey’s post-test with a 95 % confidence interval (Graphpad Prism 3.03); * = p <0.05; ** = p < 0.01; *** = p < 0.001