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Fig. 1 | BMC Cancer

Fig. 1

From: Inhibiting inducible miR-223 further reduces viable cells in human cancer cell lines MCF-7 and PC3 treated by celastrol

Fig. 1

Effects of celastrol on number of living cells and miR-223 in two cell lines. a Celastrol’s effects on viable cell numbers. MCF-7 or PC3 cells were cultured with different doses of celastrol (with solvent DMSO as control) for 24 h. Then the number of viable cells was quantified by flow cytometry with one-platform protocol, as detailed in Methods. b Inducing miR-223 with celastrol. After incubation with celastrol at the indicated doses for the indicated time, the cells’ total RNA was extracted and reverse transcription was performed. Then miR-223 levels were determined by quantitative PCR with specific primers. Cel represents celastrol. The tests were repeated three times. Data is presented as mean ± SD; statistics were determined between the celastrol-treated group and DMSO, * indicates P < 0.05, while ** indicates P < 0.01

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