Fig. 2

L2H17 suppressed cell growth of CT26.WT cells. After the CT26.WT cells were seeded in E-plates for 24 h, cells were then treated with L2H17 (a) or curcumin (b) in various concentrations (1, 3, 10, 30 or 100 μM) for 100 h. The cell growth curves were recorded and analyzed by the RT-CES system (Acea Biosciences Inc., San Diego, CA). (c) Cells were exposed to various concentrations of L2H17 (1, 3, or 10 μM) for 7 days followed by clonogenic assay. Curcumin (10 μM) or DMSO (3 μL) was added as the positive and negative controls, respectively. Visualized colonies were then photographed and calculated. The data were obtained from three independent experiments performed in triplicate. And the representative photos were shown. (d) The calculated colonies were presented as mean ± SEM. The indicated differences are significant ** P < 0.01, *** P < 0.001, and **** P < 0.0001, t-test, compared to the DMSO-treated group