Fig. 3

Expression of CIP2A in different lung cancer cell lines and effect of CIP2A in lung cancer cell proliferation. a Expression of CIP2A is evaluated in two immortalized lung cell lines, four NSCLC cell lines and two SCLC cell lines using Western-blot analysis. b The know down and overexpression effects were examined by Western-blot. In A549 and H838, CIP2A were knock down by two independent shRNAs. In H460 and H69 cell lines, full-length CIP2A were overexpressed by lentiviral transfection. The cell proliferation of these four cell lines was measured at 1st passage. c Cell proliferation was performed on four lung cancer cell lines with either knock down (shRNA1 or shRNA2) or overexpression (+CIP2A). For adherent cell lines (A549, H838 and H460), three thousands cells for each cell line were plated triplicate in 96-well plates and MTT solution was added at day 1 and day 3. For suspension cells (H69), cells were resuspended as single cell and 4000 cells were plated triplicate in 96-well plate, and cell proliferation was measured the same as adherent cell. O.D absorbance was read at 570 nm. Statistical significance was done with Students’t test