Fig. 2

Androgen responsiveness of LNCaP and LNCaP-AI cells. a Androgen responsiveness of LNCaP-AI cells was lost by 24 weeks of prolonged culture compared to parental LNCaP cells. Data are shown as % cell proliferation of DHT treated vs. no DHT treatment condition. For this proliferation assay, cells were treated with different concentrations of DHT for a period of 6 days. b No difference in cell migration was seen between parental and subline cells. However, LNCaP-AI cells were more invasive (c) compared to parental LNCaP cells. (N = 3, error bars = SEM, * indicates P < 0.05). Picture inset underneath the graphs are representative images of cell migration and invasion (cells are stained purple)