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Fig. 2 | BMC Cancer

Fig. 2

From: Mice null for the deubiquitinase USP18 spontaneously develop leiomyosarcomas

Fig. 2

Reconstitution of USP18 in USP18−/− sarcoma cell lines did not reverse tumorigenesis. a: Stable transfection of human USP18 restored USP18 deconjugase activity in the USP18 knockout KHC-1 and KHC-2 leiomyosarcoma cell lines. The ISG15 conjugates were more prominent after 24 h treatment with IFNβ. b: Monolayer growth assays of empty vector versus USP18 reconstituted cells over 72 h expressed as fold difference versus control cells. c: Anchorage-independent growth for KHC-1 and KHC-2 cell lines, with or without USP18 reconstitution. d: Tumorigenesis of KHC-1 and KHC-2 cells in athymic mice in USP18 reconstituted cells versus vector control cells. Error bars represent standard deviation

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