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Fig. 1 | BMC Cancer

Fig. 1

From: Mice null for the deubiquitinase USP18 spontaneously develop leiomyosarcomas

Fig. 1

FVB-USP18 knockout mice develop sarcomas. a: PCR genotyping assays of USP18−/−, USP18+/−, and USP18+/+ genomic DNA (null allele, 370bp and wild-type allele 151bp). b: USP18 null mice with tumors. c: H & E staining of representative sarcomas. The solid arrow indicates a multinucleate giant cell. The spindle cell characteristic of the sarcomas is depicted in the right panel and the hatched arrow indicates a mitotic cell. d: Kaplan-Meier curve is displayed depicting the times when sarcoma development necessitated euthanasia in USP18−/− mice versus USP18+/+ and USP18+/− mice, respectively. USP18 null mice significantly developed sarcomas (P < 0.0001) while no sarcomas were seen in wild-type or heterozygous mice. e: H & E staining of a representative murine leiomyosarcoma in a USP18 −/− mouse is displayed along with the expression profiles for S100, cytokeratin (AE1/AE3), desmin, calponin and SMA (smooth muscle actin). f: A representative leiomyosarcoma cell line (KHC-2) was also examined for the same markers displayed in panel E. A phase-contrast image of this cell line is provided. g: The P53 immunostaining is of two USP18−/− murine leiomyosarcomas, one positive for nuclear P53 and one negative

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