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Fig. 2 | BMC Cancer

Fig. 2

From: Ascorbic acid and ascorbate-2-phosphate decrease HIF activity and malignant properties of human melanoma cells

Fig. 2

Effect of ascorbic acid and ascorbate 2-phosphate on HIF-1α stabilization in WM9 metastatic melanoma cells. WM9 metastatic melanoma cells were treated with increasing concentrations (10, 25 or 50 μM) of ascorbic acid (AA), or the non-oxidizable analog ascorbate 2-phosphate (A2P) for 15–120 min under standard normoxic culture conditions. Western blot analysis of isolated nuclear fractions reveals that both (a) AA and (b) A2P cause nearly 50 % reduction of normoxic stabilized HIF-1α in these cells as early as 30 min following treatment. Treatment with A2P provided nearly complete reduction in stabilized HIF-1α by 120 min. Protein expression was normalized to β-actin. All treatments were repeated a minimum of 2 additional times with similar results

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