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Fig. 1 | BMC Cancer

Fig. 1

From: Ascorbic acid and ascorbate-2-phosphate decrease HIF activity and malignant properties of human melanoma cells

Fig. 1

Effect of ascorbic acid on HIF-1α stabilization in WM1366 radial growth phase melanoma cells. WM1366 cells were treated for 24 h with ascorbic acid (AA; 5–50 μM) under standard normoxic culture conditions. a Western blot analysis of isolated nuclear extracts reveals considerable reduction in the amount of stabilized HIF-1α protein following treatment with AA. b Densitometry analysis demonstrates the ability of AA supplementation at physiologically achievable concentrations to reduce the normoxic overexpression of HIF-1α by approximately 50-60 % in these cells. Protein expression was normalized to β-actin

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