Fig. 5
From: A novel aspirin prodrug inhibits NFκB activity and breast cancer stem cell properties
The effects of GTCpFE in MS culture of breast cancer cells. a MS formation and cell viability from indicated cell lines was measured after treatment with varying concentrations of GTCpFE. The effect of GTCpFE in both assays is plotted as % of DMSO vehicle control. b p65 DNA binding activity was measured in conventional adherent 2D culture of MCF-7 cells or MS culture with or without inhibitors (IKKVII 1 μM or GTCpFE 50 μM) added for the last 3 hours. c-d TNF (C) and CCL2 (D) expression after 6 hours was measured in treatment groups described in (B). The different letters (a, b, c) above bars indicate significant difference between treatments, P < 0.05. e Total and p-p65 levels are measured in 2D vs MS culture treated with 50 μM GTCpFE. f PGE2 levels in the media of MDA-MB-231 MS was measured upon treatment with 20 μM GTCpFE for 24 hours, * P < 0.05