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Fig. 4 | BMC Cancer

Fig. 4

From: A novel aspirin prodrug inhibits NFκB activity and breast cancer stem cell properties

Fig. 4

ASA cannot inhibit NFκB activity in breast cancer cells. a NFκB-RE activity or b NFκB target gene expression (ICAM1, CCL2 and TNF) was measured in MCF-7 cells treated with different concentrations of ASA. c RT-QPCR for NFκB target gene expression was measured in MDA-MB-231 cells treated with increasing concentrations of ASA. TNFα was used to activate NFκB, and ASA response is plotted as % of TNFα alone. d ASA cannot inhibit TNFα induced phosphorylation of IKKs. MCF-7 cells were pretreated with 50 μM GTCpFE or ASA for 2 hours followed by TNFα for 15 minutes. Phosphorylated and total IKK levels were examined by western blotting. β-actin served as a loading control

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