Fig. 5From: Downregulation of toll-like receptor 4 induces suppressive effects on hepatitis B virus-related hepatocellular carcinoma via ERK1/2 signalingTLR4 knockdown influences the expression and activation of multiple downstream genes. a Representative images of 3 independent experiments with similar results are shown, silencing of TLR4 decreases the phosphorylation of ERK1/2 protein and c-Fos protein, a well-defined effector of ERK1/2 signaling. However, silencing of TLR4 has no impact on JNK1/2, NF-κB or p38 signaling. P < 0.05. b Chemical inhibitors SB203580, SP600125, PD184352 and PDTC were used to block MAPKs and NF-κB activation, and LPS was used to stimulate TLR4 signaling. After indicated treatments, the cell viability was assessed at different time points using the CCK8 assay. Combined treatments with PD184352 and LPS significantly reduced the proliferation rate of HepG2.2.15 cells, compared with those treated with LPS and SB203580, SP600125 or PDTC. P < 0.05Back to article page