Fig. 5

NF-κB is important for HIF-1α expression. a The cells were transfected with BRMS1 or empty vector (control) for 48 h and then stimulated with TGF-β1 (5 ng/ml) for 6 h. Immunoblotting. b The MDA-MB-231 cells transfected with a luciferase vector containing a HIF-1α promoter were pretreated with BAY11-7082 (5 μM) for 1 h, followed by stimulation with TGF-β1 (5 ng/ml) for 24 h. Luciferase activity (*P < 0.05 vs DMSO, ^##P < 0.01 vs TGF-β1 treatment only). c The serum-starved MDA-MB-231 cells were pretreated with BAY11-7082 (5 μM) for 1 h and then stimulated with TGF-β1 (5 ng/ml) for 6 h. Immunoblotting. Representative results were presented from at least three independent experiments with similar results