Fig. 2

BRMS1 inhibits Snail and TWIST1 expression. a The cells were transfected with BRMS1 or empty vector (control) for 48 h and then stimulated with TGF-β1 (5 ng/ml) for 6 h. Quantitative RT–PCR. Relative mRNA levels normalized to the expression of the housekeeping gene, GAPDH (*P < 0.05 and ***P < 0.001 vs control, ^#P < 0.05 and ^##P < 0.01 vs control with TGF-β1). b The MDA-MB-231 cells were transfected with BRMS1 or empty vector (control) for 48 h and then stimulated with TGF-β1 (5 ng/ml) for 6 h. Immunoblotting. c and d The MDA-MB-231 cells were cotransfected with the empty vector (control), BRMS1 and indicated reporter plasmids for 48 h, followed by stimulation with TGF-β1 (5 ng/ml) for 24 h. Luciferase activity (**P < 0.01 and ***P < 0.001 vs control,^##P < 0.01 vs control with TGF-β1). Representative results were presented from at least three independent experiments with similar results