Fig. 1
From: Comparative analysis of 14-3-3 isoform expression and epigenetic alterations in colorectal cancer
Expression levels of 14-3-3 genes in tumor and non-tumor colon tissues as determined by RT-PCR. Purified whole-RNA extracts taken from frozen tissues were reverse-transcribed and quantified using real-time PCR. Each of the seven human 14-3-3 isoforms are normalized to the housekeeping gene GAPDH (ΔCt). Box plot compares expression of all seven 14-3-3 isoforms in tumor (T, n = 71) and non-tumor (N, n = 52) sample groups. Y-axis is plotted as inverse ΔCt. The dark, horizontal bar indicates sample mean and box outlines mark first and third quartiles. Whiskers extend to sample minimum and maximum. Brackets indicate statistically significant differences (determined by Student’s t-test, P < 0.001)