Fig. 3

MIF affects the colony forming ability of the GBC cells – a-i Expression of MIF across a panel of GBC cell lines. Western blot analysis was performed using anti-MIF antibody. β-Actin was used as loading control. Top panel: GBC whole cell lysates, Middle panel: β-Actin, Bottom panel: GBC cell secretome. a-ii A graphical representation of MIF expression in GBC cell lines compared to β-Actin. b-i Colony forming ability of GBC cell lines was decreased post-transfection with MIF siRNA. b-ii A graphical representation of the same *P < 0.05. c-i Inhibition of MIF in GBC cell lines with ISO-1 (50 μM) and 4-IPP (5 μM) led to a decrease in the colony forming ability of the cells. c-ii A graphical representation of the same *P < 0.05