Fig. 4

WZ35 induced cell cycle arrest in the G2/M phase in PC-3 cells. Cells were treated with WZ35 at different concentrations as indicated or curcumin (20 μM) for 24 h, then cells were processed for cell cycle analysis (a) or western blot analysis for expression of cell cycle-associated proteins (b–d) as described in methods. The statistic data were presented as mean ± S.E from three independent experiments. *, p < 0.05, **, p <0.01; ***, p <0.001; all versus DMSO group