Fig. 8
From: Targeting glycolysis by 3-bromopyruvate improves tamoxifen cytotoxicity of breast cancer cell lines
Effect of TAM, 3-BP and their combination on the extracellular level and the expression of Metastasis markers. After adding 3-BP to TAM succeeded to decrease the extracellular level of MMP-2 and 9 using gelatin zymography in MCF-7 cells (a) and T47D (b) cells. The analysis was done by image software. The effect of this combination on the secreted MMPs was reflected on their genes expression using qPCR in MCF-7 (c) and T47D (d). Results are expressed as means ± SD of 2 independent experiments for zymography but for qPCR results are expressed as means ± SD of 2 independent experiments performed in duplicates. Significance was determined with one way ANOVA using Tukey’s multiple comparison test.“a” Significantly different from control, “b” from 3-BP and “c” from TAM at P ≤ 0.05. ♦Significant interaction (antagonism) when TAM and 3-BP where combined using factorial design