Fig. 4
From: SUMOylation of sPRDM16 promotes the progression of acute myeloid leukemia
SUMOylation of sPRDM16 enhanced the engraftment of systemic THP-1 transplantation leukemia in NOD.CB17-Prkdcscid/J (NOD/SCID) mice. Sub-lethally irradiated mice were inoculated via the tail vein with 1 × 107 GFP-labeled THP-1 cells stably infected with Lenti-Vector, sPRDM16–WT or sPRDM16–K568R. Fluorescent grafts were monitored by flow cytometry. All mice were sacrificed and subjected to subsequent test 30 days post-transplantation of THP-1 cells. a FACS blots of leukemic cells (GFP+) in the femur bone marrow in mice inoculated with Lenti-Vector, sPRDM16–WT or sPRDM16–K568R THP-1 cells 30 days post-transplantation. The dot plots showed SSC (y axis) versus GFP intensity (x axis). b Bar graphs summarized the percentage of leukemic cell (GFP+) in the bone marrow per femur over whole population (n = 5-6). c Dot blots indicated leukemic cells (GFP+) in mice peripheral blood 30 days post-transplantation of Lenti-Vector, sPRDM16–WT or sPRDM16–K568R THP-1 cells. The dot plots showed SSC (y axis) versus GFP intensity (x axis). d Percentages of leukemic cells (GFP+) in mice peripheral blood over whole population (n = 5-6). e H&E staining of marrow cavity of femur section from the mice transplanted with THP-1 cells stably infected with Lenti-Vector, sPRDM16–WT or sPRDM16–K568R. f Mean animal weight