Fig. 6

Effect of Osteoprotegerin on the proliferation of HMEC spheres. a Schematic of the experiment. b Confocal images of control HMEC spheres grown in various media combinations. HMEC cells were seeded in various media in 6 well ultra-low attachment plates as indicated, grown for 8 days, and imaged (magnification, 10X). Results shown are tiled shots of two different fields and the assay was performed in triplicate. c and d OPG increases the proliferation of HMEC spheres. MTT assay results shown in the panel represent the absorption at 570 nm. HMEC spheres grown in various supernatants for 8 days as described in methods. The values correspond to the mean +/− S.D. of three independent experiments