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Fig. 2 | BMC Cancer

Fig. 2

From: Osteoprotegerin secreted by inflammatory and invasive breast cancer cells induces aneuploidy, cell proliferation and angiogenesis

Fig. 2

Cytokine array analysis of the conditioned media from (a) adherent cells and (b) sphere cultures of HMEC, SUM149PT and SUM1315MO2 cells. Red box in the panel indicates the spot on the array corresponding to OPG. c and d Semiquantitiative densitometric analysis of the OPG in the supernatants obtained from adherent cells and sphere cultures of HMEC, SUM149PT and SUM1315MO2. e Conditioned media from adherent HMEC, SUM149PT and SUM1315MO2 cells were collected and analyzed by OPG ELISA. f Lysates from adherent cells of (a) HMEC, SUM149PT and SUM1315MO2 or (b) HMEC and SUM190PT were Western blotted for OPG, stripped and immunoblotted for (a) GAPDH or (b) β-actin used as loading control. A representative blot from three independent experiments is shown

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BMC Cancer

ISSN: 1471-2407

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