Fig. 3

Pathway analysis revealed that CRTC1-MAML2 fusion induces critical cancer cell signaling. a Functional classification of fusion-regulated genes (Additional file 2: Table S1) was performed using Ingenuity Pathway Analysis (IPA). The top 20 Molecular and Cellular Functions were ranked based on p-value, and the bars represent inverse log of the p-value (x-axis). b Top 20 Canonical Signaling Pathways that were enriched in fusion-regulated genes were shown. These pathways were ranked based on p-value, and the bars represent inverse log of the p-value (x-axis). c Upstream regulators analysis identified several transcription regulators regulated CRTC1-MAML2 fusion targeted genes. Changes in the activation status of transcription regulators were plotted based on their activation z-score from IPA. Positive z-score indicates activation and negative z-score indicates inhibition. d Upstream regulators analysis identified several drugs or kinase inhibitors regulated CRTC1-MAML2 fusion targeted gene expression. e MAPK inhibitor (U0126) regulated several CRTC1-MAML2 fusion down-regulated genes (Activation z-score: 3.89; p-value of overlap: 1.65E-5), suggesting that fusion function can be blocked by the inhibition of the MAPK pathway