Fig. 1

Estrogen and progesterone induce a unique transcriptomic response in ZR-75-1 and T-47D cells. a Protein steady state levels of ERα, PR-A, PR-B, androgen receptor (AR), androgen and progesterone regulated gene FKBP5 and estrogen regulated gene CTSD in ZR-75-1, T-47D and MCF-7 cells treated with ethanol (v.c.), 10nM DHT, 10nM PROG or 10nM estrogen for 16 h. TUBA and calnexin (CANX) were utilised as controls. Note that exposure time was different for each cell line and was optimised to visualise changes in response to hormone treatment. b Non hormone treated protein steady state levels of ERα, PR-A and PR-B in ZR-75-1, T-47D and MCF-7 cells treated with v.c. for 16 h. Alpha tubulin (TUBA) was utilised as a control. Exposure times were different from the blot presented in Fig. 1a. c Microarray analysis of the transcriptomic response of ZR-75-1 cells treated with ethanol (v.c.), 10 nM estrogen, 10 nM PROG, or cotreated with 10 nM estrogen and 10 nM PROG for 16 h. Euler diagram (left) demonstrates commonly regulated genes and those uniquely regulated by the hormonal cotreatment. Histograms (right) demonstrate validation of progesterone-regulated responses in independent samples. Expression presented relative to housekeeping gene GAPDH expression (d) Microarray analysis of the transcriptomic response of T-47D cells treated with ethanol (v.c.), 10 nM estrogen, 10 nM PROG, or cotreated with 10 nM estrogen and 10 nM PROG for 16 h. Euler diagram (left) demonstrates commonly regulated genes in response to each treatment. Histograms (right) demonstrate validation of progesterone-regulated responses in independent samples. e Cell cycle analysis of propidium iodide stained ZR-75-1 cells after treatment for 24 h with vehicle (V.C; ethanol), 10nM progesterone or pretreated for 72 h with 10nM estrogen (E2p), followed by 16 or 24 h of 10nM progesterone treatment (E2p + P4)