Fig. 4

IVC as a mediator of ROCK. a MHCC97H cells were treated with increasing concentrations of IVC for 24 h and p-MYPT-1 was examined by western blot. ROCK inhibitor Y27632 was used as the positive control. b The relative protein level in each condition was quantitated using Image J. c IVC inhibits the formation of stress fibers. Starved MHCC97H cells were treated with either vehicle, Y27632 or IVC for 1 h and then fixed and stained with phalloidin (green) and DAPI (blue). Original magnification was 400×, scale bars represent 25 μm. Data are representative of three independent experiments. Data are expressed as mean ± S.E. *P < 0.05 and **P < 0.01 vs. the control