Fig. 2

IVC affects cell cycle progression. a MHCC97H cells were incubated with IVC at indicated doses for 24 h, and the DNA content of propidium iodide-stained cells was analyzed by flow cytometry. b Cell cycle distribution was analyzed. c mRNAs of cell cycle regulatory genes were detected by real time-qPCR assay. d Protein expressions of CDK-2, cyclin-E1, p21, and p53 were detected by western blot. e The relative protein level in each condition was quantitated using Image J and represented as a line chart corresponding to pixels detected. Data are expressed as mean ± S.E. (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the control