Fig. 4

NF-κB binds to NOS2 promoter to activate iNOS expression in human colon carcinoma cells. a The NOS2 gene promoter structure. The NF-κB consensus sequences are indicated. The locations of ChIP PCR primers are also indicated. b ChIP analysis of NF-κB association with the NOS2 gene promoter. c EMSA of NF-κB binding to DNA. Human colon carcinoma cells were treated as indicated and used for nuclear extract preparation. The DNA probes containing the NF-κB consensus sequence (#2 as shown in A) were incubated with the nuclear extracts and analyzed for NF-κB-DNA association. Shown are duplicated results (Replicate 1 and Replicate 2). A NF-κB control probe (Santa Cruz Biotech) was used as a positive control probe. The probe sequences are presented in Table 1