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Fig. 3 | BMC Cancer

Fig. 3

From: The NF-κB p65 and p50 homodimer cooperate with IRF8 to activate iNOS transcription

Fig. 3

IFNγ and TNFα cooperatively induce iNOS expression in human colon carcinoma cells. a Tumor cells were treated with IFNγ, TNFα, or both IFNγ and TNFα for approximately 18 h, and analyzed for iNOS expression by RT-PCR. β-actin was used as a normalization control. b Cells were treated as in A and then analyzed by Western blotting analysis of iNOS expression with β-action as an internal control. c Tumor cells were cultured in the presence of Ruxolitinib for 30 min and then treated with IFNγ and TNFα as indicated for 18 h. Total lysates were then prepared and analyzed for STAT1 and pSTAT1 levels by Western blotting analysis. d The cells were treated as in C and then analyzed by RT-PCR for iNOS expression. e The cells were transfected with either scramble siRNA or human IRF8-specific siRNA for 6 h and the cells were treated with IFNγ for 18 h. The cells were analyzed for IRF8 and iNOS expression by RT-PCR with β-actin as a normalization control

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