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Fig. 6 | BMC Cancer

Fig. 6

From: Suppression of Homologous Recombination by insulin-like growth factor-1 inhibition sensitizes cancer cells to PARP inhibitors

Fig. 6

IGF-1R inhibition reduced RAD51 expression both at mRNA and protein levels. Using HR proficient SKOV3 and BT20 cancer cells, quantitative RT-PCR analysis showed the reduction of RAD51 mRNA levels in cells treated with IGF-1Rki (a) at different times (12 h-24 h) (b) and doses (1-5 μM). c IGF-1R inhibition decreases cisplatin-induced RAD51 foci formation in these cells (>5 RAD51 foci/nucleus were considered positive). d Treatment with 5uM of IGF-1Rki for 24 h reduces the expression of RAD51 and IGF-1R pathway proteins, determined by Western blot analysis. A representative blot out of 3 is shown. e After treatment with 5uM IGF-1Rki for 24 h, clarified protein lysates from SKOV3 (e) and BT-20 (f) cells were subjected to immunoprecipitation. Precipitates were blotted against RAD51 and IRS-1. One representative blot out of 4 is shown. Results represent the average of four independent experiments. *p value <0.05

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